Journal of Animal and Poultry Sciences (JAPSC)
Vol 5(2), 2016
Salmonella: Isolation and antimicrobial susceptibility tests on isolates collected from poultry farms in and around Modjo, Central Oromia, and Ethiopia
Abunna*, M. Bedasa, T. Beyene1, D. Ayana, B. Mamo, and R. Duguma
A cross-sectional study was conducted during period of February, 2015 to May, 2015 with the objective of Salmonella isolation from poultry farms in and around Modjo town and to determine antimicrobial susceptibility profiles of the isolates. Accordingly, at total of 205 samples in which 100 cloacal swabs, 75 fresh feces, 10 litter samples, 8 chicken feed samples, 8 poultry drinking water and 4 chicken handlers’ hand swab samples were collected.31(15.12%) isolates were detected from 205 collected samples. The studied poultry farms had different prevalence rates but not statistically significant. The lowest prevalence was 5(10.64%) whereas the highest was 10 (20.00%). These isolates 11(11.00%), 14(18.67%), 4 (40.00%) and 2 (25.00%) were recovered from cloacal swabs, fresh feces, litter and poultry drinking water samples respectively. Of the 31 isolates, 21 (67.74%) were motile (contributes to zoonoses) while 10(32.26%) were non-motile. Thirty of 31 isolates were resistant to one or more of antibiotics. Of 30, 19 were multidrug resistant while 11 isolates were only resistant to tetracycline. One isolate was resistant to tetracycline and Kanamycin. Furthermore, 2, 5, 4, and 7 isolates were tetra-, penta-, hexa-, and hepta-resistant, respectively. All the 31 isolates were susceptible to Ciprofloxacin and Gentamycin. 18 (94.73%) of multi-drug resistant (MDR) isolates were found resistant to five to seven different antimicrobials. According to this finding, Salmonella was isolated from different sample type, poultry growth stage, and breeds indicating its wider distribution. The detection of multi drug resistant 61.29% (19/31) isolates and 67.74% with likely of zoonotic potential indicated the salmonellosis could be an emerging poultry and public health problem. Therefore, further research is needed on major risk factors and molecular characterization for serotyping and genomic studies to have an idea about genes responsible for pathogenecity and drug resistance of the isolates of Salmonella.
Key words: Isolates, Multidrug resistant, Motility, Poultry breeds, Poultry farms, Poultry growth stage, Sample type