2014, Number 1

  Journal of Animal and Poultry Sciences (JAPSC)

Vol 3(1), 2014

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Atypical Actinobacillosis in a Dairy Cow

G. Farjani Kish*,A. Tabatabaei Naeini, F. Namazi, ,Y. Ariyzand

1-7

Abstract

Actinobacillosis is an infectious, chronic, generally non-fatal disease caused by Actinobacillus lignieresii. The etiologic agent is a part of the oral flora and causes pyogranulomatous lesions of the soft tissues. A 5-year old, approximately 500 kg cross-breed Holstein cow was presented for the treatment of a large, ulcerated and hemorrhagic mass at the left side of the neck in vicinity of the jugular furrow. Anamnesis indicated that the condition began three months previously as a small, walnut-size swelling that gradually enlarged over this period. After surgical resection of the mass, histological assessment revealed multiple pyogranulomatous foci-contained radiating eosinophilic clubs surrounded by many neutrophils, lymphocytes, plasma cells, macrophages and connective tissue. Clinical manifestations, bacteriological and pathological examinations of biopsy confirmed atypical actinobacillosis. In this case, the route of entry of organisms probably had been an abrasion or wound in the skin of the neck.

Key words: Actinobacillus lignieresii, Pyogranulomatous, Bacteriological, Pathologic, Biopsy

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Achievement to the goat’s spermatogonial stem cells from embryos

                Alireza Hasani Bafarani, Ziaoddin Mirhoseini, Farid Heidari

8-18

Abstract

The present study reports an easy approach to obtain an abundant origin of pluripotent spermatogonial stem cell (SSC) from the goat fetal testis tissue. These cells showed tissue non-specific alkaline phosphatase activity and also express three markers of pluripotency (NANOG, SOX2 and OCT4) which are also expressed in embryonic stem cells. We found that these cells can be used for co-culturing with the inactivated goat embryonic fibroblast for several passages without differentiation or changing in shape. It was also found out that, these cells can remain viable at -70 °C one month by using DMSO.

Key Words: Fetal spermatogonial stem cells, Germline stem cells, Goat embryo

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Effect of xylanase and cellulase supplementation on growth performance, volatile fatty acids and caecal bacteria of broiler chickens fed with palm kernel meal-based diet

Sharmila, K. Azhar, M. N. Hezmee, and A. A. Samsudin*

19-28

Abstract

In this study, the effect of xylanase and cellulase supplementation in palm kernel meal (PKM) based diet on growth performance, volatile fatty acids (VFAs) and the caecal bacterial populations of broiler chickens were investigated. Seventy five day old male Cobb broiler chicks were randomly allocated to three dietary treatment groups receiving T1 (20% PKM-based diet without enzyme), T2 (20% PKM-based diet with xylanase) and T3 (20% PKM-based diet with cellulase). Each enzyme was supplemented at an amount of 200U/kg of feed. Weekly body weight gain and feed intake were recorded. All chickens were slaughtered on day 35 and the caecum content was aseptically collected for VFAs quantification and bacterial enumeration. Supplementation of xylanase and cellulase in PKM diets had different effect on the growth performance, the number of caecal bacterial population and VFAs produced. A significant reduction in the cumulative feed intake of birds fed cellulase-supplemented PKM compared to xylanse-supplemented and unsupplemented PKM diet was observed. However, the final body weights gain and cumulative feed conversion ratio (FCR) were not significantly different between the treatment groups. Determination of VFAs production of the caecal contents demonstrated a significant difference in the production of iso-butyric and n-valeric acid among treatment groups. The number of total viable bacteria, lactic acid bacteria (LAB) and coliform in caecal samples were also enumerated. Significant difference was observed in the number of caecal bacteria population between the treatment groups. The effect of xylanase and cellulase on weight gain of broiler chickens was strongly related to the feed intake rather than due to the decrease in the number of pathogenic bacteria in the caeca. Xylanase supplementation was beneficial in enhancing cumulative feed intake, weight gain and FCR of the broiler chickens, but did not entirely reduce the number of pathogenic caecal bacteria. However, cellulase supplementation reduced all parameters observed for growth performance and the number of caecal bacteria.

Key words: Xylanase, Cellulase, Palm Kernel Meal, Bacteria, Broiler

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Mycological and pathological study of broiler mortalities associated with clinically apparent respiratory diseases

Madadi M. S*,Ashrafi Helan. J , Zare. P

29-37

Abstract

In this study, 1136 tissue samples from mortalities of 230 suspected broiler farms were cultured and subjected to isolation, laboratory and histopathology diagnosis of Fungi species. Four fungi species were isolated from respiratory system of necropsied broilers; they were Aspergillus fumigatus, Mucor spp, Aspergillus niger and Aspergillus flavus. At necropsy, extensive yellowish white caseous miliary nodules in the lung and air sacs were seen. Histopathologic examination of tissue sections revealed granulomas in lungs and thickened abdominal air sac membranes. The results of this study indicated that fungal species are present in birds with apparent respiratory diseases. It is very important to be aware of the high prevalence of these organisms, the sources and points at which the chicks become infected with the organisms as well as the diseases they can cause and possibly put them under surveillance as important pathogens of poultry.

Key words: Fungal, Broiler, Isolation, Histopathology, Mortality