2013, Number 3

  Journal of Animal and Poultry Sciences (JAPSC)

Vol 2(3), 2013


On farm evaluation of group treatment of digital dermatitis in dairy cows

I. Nowrouzian, M. Nouri*, F. Zibaee, and F. Katouli



The aim of this blind and longitudinal farm study was to evaluate the efficacy of Provita Hoofsure Endurance (PHE) foot wash solutions in reducing the prevalence of lameness due to digital dermatitis (DD) in dairy cows. Cows (n = 182) from three commercial dairy herds of closed type were diagnosed by lameness and pain scoring, with lesions consistent with DD in different stage of development. On the basis of locomotion scoring system (1-5), the prevalence of lameness was between 28.8% and 43.6%. Cows were considered for individual evaluation based on significant Kappa-value and restrained in a chute for lesion type (0-4), color (0-2) and size (0-2) scoring prior to application of PHE and at the end of trial in walk-through footbaths or group topical spray in milking parlor. Cows were allocated to one of two groups: cows in group 1 were treated with PHE 2.0% twice a day for 3 consecutive days in footbath, and treatment repeated again after 2 weeks with PHE 1.0% twice a day for 3 consecutive days. In group 2, 2.0% solution was sprayed on the palmar surface of the feet. Changes in each score between initial and final scoring were calculated and the comparison was made statistically using non-parametric U-test with 0.05 level of significance. From the results of this farm study, it is concluded that the application of PHE alleviates lameness significantly at the herd- or the cow-level as part of a control program of DD.

Key words: digital dermatitis, cow, lesion, footbaths, group topical spray


Radiological study of the effect of Platelet Rich Plasma (PRP) on experimentally induced Osteoarthritis in Guinea Pig’s stifle joint

N. Seydi, and S. N. Dehghani*



The purpose of this radiologic, longitudinal study was to estimate the effects of the platelet rich plasma (PRP) on improvement of the defective articular cartilage in an osteoarthritis (OA) model. The PRP is an autologous product that concentrates a high number of platelets in a small volume of plasma. It mimics the last step of the coagulation cascade, leading to formation of a fibrin clot, which consolidates and adheres to the application site in a short period of time. Twenty adult guinea pigs (treatment group, n = 10; control group, n = 10) were anesthetized and the anterior cruciate ligament of the left knee was transected through a para-patellar approach. The animals were allowed to resume normal cage activity for 12 weeks. For the preparation of PRP, 2 ml blood was collected into a Na-citrate tube by direct heart puncture. Twelve weeks post-operation PRP was injected into the OA joint of the treatment group only. The animals were observed for further 8 weeks. At 20 weeks radiological score of OA increased in control group. Reduction in the degree of osteophyte formation, and subchondral bone sclerosis were detected in the PRP-treated joints. In conclusion, this study shows beneficial effects of the PRP on improvement of the defective articular cartilage in the OA joint.

Keywords: Platelet rich plasma, Osteoarthritis, Stifle joint, Guinea pigs, Radiological study


Molecular and phylogenetic characterization of Ornithobacterium rhinotracheale isolates from turkey, quail, partridge and domestic pigeon in Iran

S. Mirzaie*, and M. Hassanzadeh



Ornithobacterium rhinotracheale (ORT) is a respiratory pathogen which has been isolated throughout the world from numerous bird species. The present study was designed to investigate molecular and phylogenetic characterization of ORT isolates from turkey, quail, partridge and domestic pigeon. For this purpose, one bacterial strain from each bird species which was isolated in a previous work was compared with chicken and some other bird species isolates. Isolates were streaked on 5% sheep blood agar containing gentamicin. Genomic DNA was extracted from fresh cultures by boiling of bacteria suspension. PCR was performed by using of specific primers for partial amplification of 16S rRNA gene. PCR products of ORT isolates were sequenced in both directions by an automatic sequencer. Our ORT isolates showed high identity (98.1% to 100%) in sequence of 16S rRNA gene to related data in GenBank. Phylogenetic analysis of these sequences showed that Iranian turkey and pigeon isolates were classified into a distinctive cluster with pigeon isolates from Taiwan, while Iranian quail and partridge isolates showed more relationship with the native isolates of chicken as well as some foreign isolates from different sources in the GenBank.

Keywords: Ornithobacterium rhinotracheale, turkey, quail, partridge, pigeon, PCR


Laemobothrion maximum (chewing lice) in Iranian Golden Eagles 

H.R. Azizi, M. Adel*, E. Sayahi, A.K. Zamani Moghadam, A. Esmailian Dehkordi, M. Hematzadeh



     The Golden Eagle species have a wide geographical distribution in Iran. Some species of these birds are native and living in many aeries of Iran with different distribution. This study was carried out on 26 Iranian Golden Eagle that were referred for treatment to the poultry clinic of the veterinary faculty, university of Shahrekord. Twenty eight chewing-lice specimens were collected on the body surfaces of the birds. The parasites were fixed in a 75 % filtered ethanol solution, cleared in 85 % lactic acid and mounted on slides using Hoyer fluid. They were mounted on slides separately after being cleared in lactophenol. Laemobothrion maximum were identified by microscopically examination, also one abnormal shape of this lice was observed.

Key Words: Laemobothrion maximum, Iranian Golden Eagles, lice.